Figure 5: IgG production in serum from infected mice immunized with LevRad associated or not to fluconazole. Antibody responses against Mexo were determined just before (P0), as well as at 30 (P30), 60 (P60) and 120 (P120) days after infection by ELISA. Negative control (C-) uninfected mice; positive control (C+) infected mice with virulent non-irradiated yeast cells of P. brasiliensis; infected mice treated with fluconazole (InMed); infected mice treated with LevRad (InRad); infected mice treated with LevRad associated with fluconazole (InRadMed). The same experiments were repeated twice with similar results. Data were reported as mean + standard deviations of optical density (450 nm) at 1:100 serum dilution in each experimental group (n=5).