Figure 6: IgG isotypes production against Mexo in serum from infected mice immunized with LevRad associated or not to fluconazole. Antibody responses against Mexo were determined 60 (A) and 120 (B) days after infection (30 and 90 days after initiating treatment) by ELISA. Negative control (C-) uninfected mice; positive control (C+) infected mice with virulent non-irradiated yeast cells of P. brasiliensis; infected mice treated with fluconazole (InMed); infected mice treated with LevRad (InRad); infected mice treated with LevRad associated to fluconazole (InRadMed). The same experiments were repeated twice with similar results. Data were reported as mean + standard deviations of optical density (450 nm) at 1:100 serum dilution in each experimental group (n=5). *** significant (p<0.001) difference in relation to C- and the other isotypes (IgG2a, IgG2b and IgG3); ### significant (p<0.001) difference in relation to C-, C+, InMed and InRadMed; &&& Significant (p<0.001) difference in relation to C- and C+ and InMed.