Immunogen Chigger Challenge Survival¬† rate
Expt. 1 Group A Montanide Uninfected chigger 10/10
Montanide Lc-1 chigger 0/10
Group B r56Lc-1+Montanide Uninfected chigger 10/10
r56Lc-1+Montanide Lc-1 chigger 3/10
Expt. 2 Group A Montanide Uninfected chigger 10/10
Montanide Lc-1 chigger 0/10
Group B r56Lc-1+Montanide Uninfected chigger 10/10
r56Lc-1+Montanide Lc-1 chigger 3/10
Expt. 3 Group A Montanide Uninfected chigger 10/10
Montanide Lc-1 chigger 0/10
Group B r56Lc-1+Montanide Uninfected chigger 10/10
r56Lc-1+Montanide Lc-1 chigger 2/10
Table 1: Evaluation of r56Lc-1 in chigger challenge model. ICR mice were immunized with either 25μg r56Lc-1 emulsion (Montanide) in the presence of CpG or adjuvant only before being fed on by either control chiggers or Orientia infected Lc-1 chiggers. Survival rates from 3 independent experiments are shown.