Figure 4: Antigen-specific long living plasma cells in the bone marrows of BALB/c mice treated or untreated for 8 days with RA (20 μg/dose/day) or its vehicle (sesame oil), intranasally immunized with TT (1 μg/dose) and sistemically (i.p.) boosted with Ag in presence of Alum after 8 months. Tetanus-specific IgG (A) and IgA (B) antibody secreting cells (ASC) were analysed by ELISPOT assay. Bars indicate the SEM of three independent experiments and stars (*) indicate that the differences between the group treated with RA and its vehicle are significant (p< 0.05).