Figure 2: T cell responses induced by DC-LLO and DC-GAPDH vaccines in listeriosis susceptible and resistant mice. A, T cell proliferation in popliteal lymph node homogenates after hind footpad inoculation of C57BL/6 mice (black bars) or Balb/c mice (grey bars) with 1×105 of different DC-LLO and DC-GAPDH vaccines prepared as in Figure 1A or saline inoculation (NT) for 7 days. Cells from DC-LLO vaccine inoculations were stimulated in vitro with 50 μg/ml of a mixture of LLO91-99 and LLO296-304 peptides and cells from DC-GAPDH vaccine inoculations were stimulated with a mixture of GAPDH1-15 and GAPDH1-22 peptides to analyse CD8 T cell proliferation. DC-controls and NT samples were stimulated in vitro with both peptides mixture. Results show the mean ± SD of [3H]-thymidine incorporation in triplicate samples (P<0.05). B, C57BL/6 (black bars) or Balb/c mice (white bars) were immunised i.p with 5×103 CFU of LM/mice for 7 days and next, left hind footpads were inoculated with 1×106 cells of different DC-LLO and DC-GAPDH vaccines or saline (NT) for 48 hours, while right hind footpads were not inoculated and served as controls. Footpad swelling was measured with a calliper and expressed as the differences in mm between left and right hind footpads. Results are expressed as the mean ± SD of three different experiments (P<0.05).