MHC-I binding H-2 allele Percentile rank Binding ability Sequence
LLO91-99 H-2-Kb
H-2-Kd
H-2-Ld		 67.5
0.7
38.3		 UB
GB
UB		 Complete
Complete
Complete
LLO296-304 H-2Kb
H-2Kd
H-2Ld		 0.2
32
12.5		 GB
UB
UB		 Complete
Complete
Complete
GAPDH1-22 H-2-Kb
H-2-Kb
H-2-Kd
H-2Kd
H-2-Ld
H-2Ld		 1.6
3.3
4.7
5.1
2.3
3.4		 GB
WB
WB
WB
IB
WB		 5-15*
5-13*
8-16
2-13
8-18
1-10
GAPDH1-15 H-2-Kb
H-2Kd
H-2Ld		 3.3
5.1
3.4		 WB
WB
WB		 5-13*
2-13
1-10
MHC-II binding H-2 allele Bindingthreshold Binding ability Sequence
LLO190-201 H-2-IAb
H-2-IAd		 75.1*
>15000		 WB
UB		 Complete
LLO189-200 H-2-IAb
H-2-IAd		 85
11364		 WB
UB		 Complete
LLO189-201 H-2-IAb
H-2-IAd		 51.3
5326		 WB
WB-UB		 Complete
Complete
GAPDH1-22 H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAd		 69.15*
70.23
70.91
71.11
74.95*
75.61
3840		 WB
WB
WB
WB
WB
WB
WB-UB		 4-18*
5-19
2-16
3-17
8-22*
7-21
2-16
GAPDH1-15 H-2-IAb
H-2-IAd		 75.72
3240		 WB
WB-UB		 Complete
Complete
Table 2: H-2 binding force predictions for LLO and GAPDH epitopes. Predictions of binding of peptides to MHC molecules performed with the IEDB analysis source Consensus tool. The lower the percentile ranks obtained, the better the binders. GAPDH1–22 peptide was compared to the predictions of LLO91–99 binding to MHC-I molecules and LLO189–201 binding to MHC-II molecules. *Comparative peptide sequence with similar binding percentiles as LLO peptides. Binding abilities: GB, good binder; WB, weak binder; UB, unable to bind. H-2 binders percentile ranks, <1.0: GB, <2.5: IB, <5: WB and >10:UB (correspond to IC values of <50 nM: high affinity, <500: intermediate affinity, <5000: low affinity). MHC-II binding thresholds, <50: GB, <500: WB, >5000: UB.