Vaccination type % Gated dimer-CD8/ peptide (H-2Kb: Ig dimer) % Gated dimer-CD8/ peptide (H-2Ld: Ig dimer)
DC-LLO91-99 2.16 ± 0.01 3.02 ± 0.02
DC-LLO296-304 0.87 ± 0.01 0.03 ± 0.01
DC-GAPDH1-15 4.06 ± 0.01 4.02 ± 0.01
DC-GAPDH1-22 4.26 ± 0.02 4.22 ± 0.03
Table 3: Frequencies of LLO and GAPDH peptides-specific CD8+ T cells induced by DC vaccines. Splenocytes from vaccinated mice were incubated with recombinant dimeric H-2Kb: Ig or H-2Ld: Ig fusion proteins loaded with LLO91-99, LLO296-304, GAPDH1-15 or GAPDH1-22 peptides. The staining cocktail contained the dimeric fusion protein loaded with the peptides, CD8 and IFN-antibodies. CD8+ cells were gated for anti-IFN-γ staining (% Gated dimer-CD8) to calculate the frequencies of CD8+-LLO91-99, CD8+-LLO296-304, CD8+- GAPDH1-15 or CD8+-GAPDH1-22 restricted cells and IFN-γ producers.