Figure 4: Ouchterlony double Immunodiffusion assay. A: Negative control; B: Guinea pig serum of SCM Originated Purified pooled material of 300mM fractions; C: Guinea pig serum of SFM Originated Purified pooled material of 300mM fractions; D: Guinea pig serum of Local reference (HO23) rabies vaccine; S: Chromatographically purified fractions (second run, 300 mM) of Rabies antigen.