Chromatography
Purification
Status
Sample
HAU
per
0.2 ml
Total protein
µg/ml
SRIDUnitage
Level of impurities
Host cellular BSA
Host cellular DNA
Before*
TCAR- CON (SCM)
16.5
37
No visible zone
>500ng/mL
10 ng/mL
TCAR- CON (SFM)
20
39
No visible zone
>500ng/mL
10 ng/mL
After*
TCAR -CON (SCM)
1442
736
7.94
Below 10 ng/mL
0.01ng/mL
TCAR -CON (SFM)
1532
755
8.0
Below 10 ng/mL
0.01ng/mL
Table 3:
Status of the rabies virus after purification. *Results are the mean values of 3 separate experiments. TCAR: Tissue Culture Anti Rabies, CON: Concentrated rabies viral harvest; SCM: Serum Contained Media; SFM: Serum Free Media.