Chromatography Purification Status Sample HAU per 0.2 ml Total protein µg/ml SRIDUnitage Level of impurities
Host cellular BSA Host cellular DNA
Before* TCAR- CON (SCM) 16.5 37 No visible zone >500ng/mL 10 ng/mL
TCAR- CON (SFM) 20 39 No visible zone >500ng/mL 10 ng/mL
After* TCAR -CON (SCM) 1442 736 7.94 Below 10 ng/mL 0.01ng/mL
TCAR -CON (SFM) 1532 755 8.0 Below 10 ng/mL 0.01ng/mL
Table 3: Status of the rabies virus after purification. *Results are the mean values of 3 separate experiments. TCAR: Tissue Culture Anti Rabies, CON: Concentrated rabies viral harvest; SCM: Serum Contained Media; SFM: Serum Free Media.