Mice immunized with various amount of rRBD (µg) |
Anti-rRBDIgGtitersa |
Neutralization Titerb |
TcdB |
TcdA |
0 |
Pre-immune |
<100 |
<4 |
<4 |
2 doses |
<100 |
NP |
NP |
3 doses |
<100 |
<4 |
NP |
3 |
Pre-immune |
<100 |
<4 |
<4 |
2 doses |
400 |
NP |
NP |
3 doses |
800 |
16 |
16 |
10 |
Pre-immune |
<100 |
<4 |
<4 |
2 doses |
400 |
NP |
NP |
3 doses |
3200 |
64 |
16 |
TcdARBDc |
3 × 10 µg |
800 |
<4 |
256 |
aThe titers were obtained with sera pooled from 6 mice
bThe neutralization titer against toxins was defined as the highest sample dilution which could prevent 50% cell rounding induced by toxins
cThe immunogenicity studies of TcdA RBD will be published elsewhere
*NP means not performed |
|