Figure 6: Antigen-specific splenic T cell cytotoxicity. Splenocytes were isolated from Trial-3 mice 2 weeks post the third vaccination. Splenic antigen-specific cytotoxic activity was determined using a chromium release assay with non-infected and IFV-infected 3T3 cells as target cells at four Effector:Target ratios. There were 6 groups of mice, PBS control (?), 10V (?), 1V (?), 1V-Al (?), 1VPIM2 (★) and 1V-Al-PIM2 (⊕). Data are expressed as mean ± SEM (n=6). Means that do not share a letter are significantly different (p<0.05, Fisher’s pairwise comparison).