PBS CT (1 µg) rVTX1 (1 µg) (3 µg) (5 µg) Weight increase (mg) 2.7 ± 7.4 55.0 ± 10.5* -2.7 ± 1.1 -2.5 ± 4.9 6.0 ± 4.2 Volume increase (μL) -12.5 ± 5.0 53.3 ± 23.1* -15.0 ± 5.0 -12.5 ± 3.5 -7.5 ± 3.5

Table 1: Toxicity of rVTX1 in ligated ileal loop in mice. Intestinal ligated ileal loop were practiced in BALB/c mice (n=3) which were inoculated with 50 μl of three different concentrations (1 μg, 3 μg or 5 μg) of rVTX1. The data correspond with the increase values compared with untreated loops (ileal loop´s weight and volume, 3 h after the inoculation). CT (1 μg) was used as positive control, and PBS as negative control. Significant differences between treatments are indicated by asterisks (*p. 0.05).