Titer 3 days after the first immunization Titer 3 days after the second immunization Titer 3 days after the third immunization Titer 3 days after the fourth immunization Rabbit No 1 Without titer At 1:50 dilution good positive slide agglutination At 1:400 dilution good positive slide agglutination At 1:400 dilution very good positive slide agglutination Rabbit No 2 Without titer Without titer At 1:50 dilution very good positive slide agglutination At 1:400 dilution very good positive slide agglutination Rabbit No1-Immunized with antigen treated with hybrid material. Rabbit No 2-Immunized with antigen treated with heat.

Table 1: Determination of the specified antibody against E.coli O 104 during and after the end of immunization scheme.