Figure 3: Neither neutralizing antibodies nor cross-reactive T cells reflect heterosubtypic protection induced by H1-DNA/SV09 and 3xSV09. Characterization of humoral and cellular immune response induced by 3x Inflexal 2009 (3xSV09), 3x H1 HA DNA followed by a boost of Inflexal 2009 (H1-DNA/SV09) or 4x PBS (PBS), 4 weeks after final immunization (n=8). A) Neutralizing Ab titer against H5N1 A/Hong Kong/156/97 and H1N1 A/Brisbane/ 59/07. B) INF-γ secreting T cells measured in ELISPOT against a pool of 215-mer peptides carrying conserved epitope; IYSTVASSL and a pool of 15-mer peptides of HA of H1N1 A/Brisbane/59/07. Group medians are shown. High titers of antibodies against NP, but not against H5 HA or NA, are elicited with both vaccination regimens and reflect observed difference in heterosubtypic protection.