Description Vaccine group Day 0 21 42 63 91d,e PBS PBS PBS PBS PBS Challenge SV09       SV09c Challenge 3xSV09   SV09c SV09c   Challenge H1-DNA   H1- DNAb H1- DNAb H1- DNAb Challenge RSV-DNA/SV09 RSV-DNAa RSV-DNAa RSV-DNAa SV09c Challenge H1-DNA/SV09 H1- DNAb H1- DNAb H1- DNAb SV09c Challenge aRSV-DNA: pcDNA2004 (Neo-) containing RSV-F-AS cDNA insert. Dose: 15 µg i.m./immunization bH1-DNA: pcDNA2004 (Neo-) containing H1 A/Brisbane/59/07 cDNA insert. Dose: 15 µg i.m./immunization CSV09: Seasonal Influenza vaccine ,Inflexal® V, of season 2009-2010. Dose 3 µg HA/strain/ immunization dFor immunogenicity experiments mice were sacrifised at day 91 and serum and spleens were collected. For influenza challenge experiments mice were challenged at day 91 eChallenge: 25xLD50 of H1N1A/Puerto Rico/8/34, H1N1 A/WSN/33 or H5N1 A/Hong Kong/156/97

Table 1: A schematic overview of the immunization regimens for the various vaccine groups is presented. The constructs used in these experiments were based on the pcDNA2004 (Neo-) plasmid backbone containing either RSV-F-A2 or H1 A/Brisbane/59/07 cDNA inserts.