Figure 3: DNA vaccine characterization. HEK293 cells were transfected with DNA vaccines, harvested after 24 h and evaluated by western blot using anti-gD (a) or sera containing anti-MAGE (b), anti-EGFRvIII (c) or anti-GLEA (d). Negative control cells were transfected with empty pVAX vector. MW: PageRuler Prestained Protein Ladder. a) Western blot using anti-gD antibody. 1, cell extract from pVAX transfection; 2, cell extract from pVAXgDMAGE transfection; 3, cell extract from pVAXgDEGFRvIII transfection; 4, cell extract from pVAXgDGLEA transfection. b) Western blot using anti-EGFRvIII antibody. 1, cell extract from pVAX transfection; 2, cell extract from pVAXEGFRvIII transfection; 3, cell extract from pVAXgDEGFRvIII transfection. c) Western blot using anti-MAGE antibody. 1, cell extract from pVAX transfection; 2, cell extract from pVAXMAGE transfection; 3, cell extract from pVAXgDMAGE transfection. d) Western blot using anti-GLEA antibody. 1, cell extract from pVAX transfection; 2, cell extract from pVAXGLEA transfection; 3, cell extract from pVAXgDGLEA transfection. The arrows showed recombinant proteins.