Figure 4: Humoral immune response induced by DNA vaccines. Sera from vaccinated mice with three doses of 100 μg of DNA vaccines were collected 20 days after the last immunization and tested for the presence of IgG2a antibodies against recombinant proteins by ELISA. a) Anti- EGFRvIII detection. The data presented are from one experiment (6 mice per group). b) Anti-MAGE detection. Representative data presented are from one experiment (6 mice per group). c) Anti-GLEA detection. The data presented are from two experiments (12 mice per group). *p<0.05, one-way ANOVA and Tukey’s posttest.