Figure 6: Assessment of cellular immune responses induced by DNA vaccines by measuring IL-10 production. Mice were vaccinated with three doses of 100 μg of DNA vaccines. Spleen cells were collected 20 days after the last immunization and stimulated ex vivo for 48 h. IL-10 production levels in the culture supernatants were quantified by ELISA. a) Culture stimulated with EGFRvIII protein. The data presented are from two experiments (12 mice per group). b) Culture stimulated with MAGE protein. The data presented are from two experiments (12 mice per group). c) Culture stimulated with GLEA protein. The data presented are from two experiments (12 mice per group). The differences in the cellular responses were determined by one-way ANOVA and Tukey’s posttest. *p<0.05, **p<0.01. The negative control was performed without any stimulus, and if detected, cytokine production was discounted for data tabulation of other stimuli. The positive control was performed using ConA as the polyclonal stimulus.