Figure 1: The sequence`and structure of the chimeric protein PSPF. A) Schematic representation of the immunogenic domains in the protein molecule1-16 T7-tag (from pET24b+), 17-185 N-terminal fragment 34 of flagellin FliC (green), 186-197 GC-flexible bridge, 198-292 C-terminal fragment of flagellin FliC (green), 293-296 GCflexible bridge, 297-402 PspA (blue), 403-406 GC-flexible bridge, 407-474 Spr1875 (brown), 475-479 GC-flexible bridge, 480-552 PsaA (red), 553-560 His-tag (from pET24b+), Flexible bridges and tags are underlined, In PSP protein the amino acids 17-296 were deleted, The predicted 3D- structure of PSPF shown in α-helixes and β-layers.