Figure 4: Immunogenicity of chimeric proteins in BALB/c mice. A) Stimulation of immune response by PSPF or PSP with or without Salmonella flagellin fragments Mice (n=10) were immunized subcutaneously with the chimeric proteins PSPF and PSP in doses 20 μg and three weeks later with 10 μg. Specific anti-PSP IgG titers on says 35, 42, 49 and 70 were assessed. Results are shown as mean ± SD. *Differencies between the groups immunized by PSPF or PSP were statistically significant on 35, 42 and 49 days after immunization. B) Immunogenicity 95 of PSPF protein in different dosage. Mice (n=10) were immunized subcutaneously with the chimeric protein in doses of 5, 10 and 20 μg and three weeks later with one-half of each dose (boost). Specific IgG titers on days 21, 35, 42, 49 and 70 were assessed. Results are shown as mean ± SD.