| Day |
Action |
| Day 0 |
The first vaccination: groups 1-3, 5
Adjuvant injection in control group: group 4 |
| Day 21 |
Taking the blood from mice to identify specific antibodies (IgG) titer |
| Day 22 |
The second vaccination (½of the first vaccination dose): groups 1-3, 5 Adjuvant injection in control group: group 4 |
| Day 35 |
Taking the blood from mice to identify specific antibodies (IgG) titer |
| Day 42 |
Taking the blood from mice to identify specific antibodies (IgG) titer |
| Day 49 |
Taking the blood from mice to identify specific antibodies (IgG) titer |
| Day 50 |
Intranasal infection with S.pneumoniae strain 73 serotype 3 |
| Day 53 |
Intraperitoneal infection with S.pneumoniae strain 442 serotype 19F |
| Days 54-63 |
Monitoring of the animals survival |
