Sample No. Sex Titer IU/ml Recommendations 1 Male < 5 < 0.043 Recommendation boost 2 Female 6000 52 Adequate 3 Male 2200 19 Adequate 4 Female 45 0.39 Consider boost 5 Male 1200 10 Adequate 6 Male 1100 9.6 Adequate 7 Female > 390625 >3396 Adequate 8 Female 21 0.18 Recommended boost 9 Female < 5 < 0.043 Recommended boost 10 Male 15625 >136 Adequate 11 Female < 5 < 0.043 Recommended boost 12 Male 1900 16 Adequate 13 Male 145 1.3 Adequate 14 Male 230 2 Adequate 15 Male 1100 9.6 Adequate 16 Female 1100 9.6 Adequate 17 Male 5100 44 Adequate 18 Female < 5 < 0.043 Recommended boost 19 Male 1500 13 Adequate 20 Male 1300 11 Adequate 21 Female 280 2.4 Adequate 22 Male 1300 11 Adequate 23 Male 3125 50 Adequate 24 Male 250 8.6 Adequate 25 Female 270 4.3 Adequate 26 Male 3600 58 Adequate 27 Male 1300 21 Adequate 28 Female 70 1.1 Adequate 29 Male 7 0.11 Recommended boost 30 Male 1800 29 Adequate 31 Male 4200 67 Adequate 32 Male 4600 74 Adequate 33 Male 4200 67 Adequate 34 Female 270 4.3 Adequate 35 Male 2700 43 Adequate 36 Male 1900 30 Adequate 37 Male 110 1.8 Adequate 38 Male 1100 18 Adequate 39 Female 450 8.6 Adequate 40 Male 12000 192 Adequate 41 Male 1300 21 Adequate 42 Male 5100 82 Adequate 43 Male 210 3.4 Adequate 44 Male 1900 30 Adequate

Table 1: Result of RFFIT of human serum samples collected from Nepal in 2014.