Figure 1: A) Analysis of T-cell proliferation in mice immunized with various forms of C. neoformans total cell lysate. T-cells were obtained from spleen of mice immunized with different vaccines formulations. The spleen T cells were stimulated with a mixture of cAg with different Conc. (0.001-500 μg/ml). After 72 hr cultivation, the proliferation of T-cells was determined by [3H]-thymidine incorporation. [H3] Thymidine incorporation was measured using liquid scintillation counter. Cells primed with PBS alone and killed yeast cells served as the control. Each point in the graph represents three determinations ± S.D. B) Analysis of T-cell proliferation in mice immunized with different forms of vaccine in response to whole cell lysate of macrophages infected with live C. neoformans. T-cells were obtained from spleen of mice immunized with different vaccines formulations. The spleen T cells were stimulated with a mixture of cAg with different Conc. (0.001-100 μg/ ml). After 72 hr cultivation, the proliferation of T cells was determined by [3H]-thymidine incorporation. [H3] Thymidine incorporation was measured using liquid scintillation counter. Cells primed with PBS alone and killed yeast cells served as the control. Each point in the graph represents three determinations ± S.D.