Figure 3: Cytokine response in mice immunized with various forms of vaccine when primed with whole cell lysate of macrophages infected with live C. neoformans. Culture supernatant of the splenocytes derived from different immunized groups was analyzed by ELISA method. The level of IL-4, IL-2, IL-12 and IFN-γ were expressed as pg/ml. Each bar represents the mean of three determinations ± S.D.