Figure 5: A) FACS analysis of splenocytes for the expression of co-stimulatory molecules. Co-stimulatory molecules on the surface of splenocytes were determined using FACS. The spleenocytes were stained with anti-CD80 and anti-CD86 molecules in triplicate. FITC-labelled anti-CD80 and anti-CD86 were used as the isotype control. B) Evaluation of number of cells containing surface marker CD4+ and CD8+ present on T-cytotoxic cells in the mice immunized with the various formulation. The percentage of surface marker CD4+ and CD8+ present on T population of the animals immunized with Synvaccine (A); Allovaccine (B) and Xenovaccine (C) are shown. C) FACS analysis of splenocytes for the expression of memory markers. Spleenocytes cells isolated from mice vaccinated with different formulation exhibited a typical effector memory (CD44high CD62Llow) as well as central memory phenotype (CD44high CD62Lhigh).