| Virus |
Primer name and sequence |
Target |
Annealing temprature |
Product size (bp) |
Reference virus |
| PCV2 |
PCV2F: 5’-AAGGGCTGGGTTATGGTATG-3’
PCV2R: 5’-CGCTGGAGAAGGAAAAATGG-3’ |
ORF2 |
55°C |
537 |
AY660574 |
| PPV |
PPV1: 5’-GAATAGGATGCGAGGAAAG-3’
PPV2: 5’-GTGGAAATCTGAGAGTCTGT-3’ |
NS-1 |
50°C |
271 |
PPU44978 |
| PRV |
PRVB1: 5’-GAAGGGGTTGGACAGGAAGGAC-3’
PRVB2: 5’-TGGGGAGCGGGTACGTGTACTA-3’ |
gB |
61.5°C |
194 |
BK001744 |
| PRV |
PRVE1: 5’-GCCACCATCGCAGAGGAACAA-3’
PRVE2: 5’-CCCACCGCCACAAAGAACAC-3’ |
gE |
61.5°C |
747 |
BK001744 |
| PRRSV |
PRRSV1: 5’-GCCAGTTCCAGCCAGTCAATCA-3’
PRRSV2: 5’-GCCCCGATTGAATAGGTGAC-3’ |
N |
58.6°C |
434 |
AF331831
AY032626 |
| CSFV |
CSFV1: 5’-CTGGCGGGTGTTATAGAGTGC-3’
CSFV2: 5’-ATACTCGCCCTTGAGCATAT-3’ |
C |
55°C |
500 |
AY775178 |
| SIV |
SIV1: 5’-ACTAAGGGCTTTCACTGAGG-3’
SIV2: 5’-ACCGTGTTACCATTCCATTC-3’ |
NS |
48.5°C |
138 |
AY700216
DQ145543
EF140698 |
| Randrom 6 mers |
5’-(p)NNNNNN-3’ |
|
|
|
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