Figure 1: Detection of T. gondii B-1 gene in adult females injected with T. gondii tachyzoites or a control PBS into hemocoel. 531-bp fragments of T. gondii B-1gene amplified by nested-PCR. The arrowhead indicates the position of the expected band. Lane M, molecular marker; lane N, negative control (ticks 10 days after PBS injection); lane 1, ticks 2 days after T. gondii injection; lane 2, ticks 4 days after T. gondii injection; lane 3, ticks 7 days after T. gondii injection; lane 4, ticks 10 days after T. gondii injection; lane P, positive control (T. gondii DNA sample).