Dose (mg/mL) 
Parameter 
Parameter Estimate 
Standard error 
P value^{a} 
0 
β_{0}^{b} 
8.543 
0.126 
<0.0001 
0 
β_{1}^{c} 
0.249 
0.024 
<0.0001 
0 
β_{2}^{d} 
0.007 
0.001 
<0.0001 

Time to max^{e} 
18.6 



Value at max^{f} 
10.9 


0.3 
β_{0} 
8.358 
0.130 
<0.0001 
0.3 
β_{1} 
0.242 
0.025 
<0.0001 
0.3 
β_{2} 
0.008 
0.001 
<0.0001 

Time to max 
14.07 



Value at max 
10.06 


0.6 
β_{0} 
8.285 
0.139 
<0.0001 
0.6 
β_{1} 
0.211 
0.027 
<0.0001 
0.6 
β_{2} 
0.007 
0.001 
<0.0001 

Time to max 
13.31 



Value at max 
9.69 


1.2 
β_{0} 
8.280 
0.181 
<0.0001 
1.2 
β_{1} 
0.091 
0.036 
0.0136 
1.2 
β_{2} 
0.004 
0.001 
0.0049 

Time to max 
10.68 



Value at max 
8.76 


2.4 
β_{0} 
8.690 
0.549 
<0.0001 
2.4 
β_{1} 
0.188 
0.107 
0.1053 
2.4 
β_{2} 
0.005 
0.004 
0.2034 

Time to max^{g} 
16.9 



Value at max^{g} 
10.28 


^{a} Significance of parameter estimates represents appropriate fit of model (Y = β_{0}
+ β_{1} x +β_{2} x^{2} + ε_{1}) to data. ^{b} β_{0} represents yintercept at time of inoculation; ^{c} β_{1}
represents the rate of bacterial growth over time; ^{d} β_{2} represents point of inflection
where growth reached its maximum at a specific time; ε_{1} represents the random
error; ^{e} Time to max = time (h) when maximal growth was achieved; ^{f} Value at max
= amount of growth (estimated log CFU) at the time when maximal growth was
achieved; ^{g} for 2.4mg/mL dose, the time to maximum growth and its value for this
treatment should be interpreted with caution due to the lack of fit indicating that the
reduced model did not fit the data for 2.4 mg/mL PGF_{2α} treatment. The lack of fit at
this specific concentration relates to the continuous decrease in bacterial growth
associated with the bactericidal effects of 2.4 mg/mL PGF_{2α} treatment. 