Table 1

Time		total	free	bound	f/b ratio	% free	% bound
Time		total	free	bound	f/b ratio	% free	% bound
8	mean	0.0	0.0	0.0	0.0	0.0	0.0
8	SD	0.0	0.0	0.0	0.0	0.0	0.0
10	mean	8.6	3.4	5.2	0.7	39.5	60.5
10	SD	0.6	0.2	0.5	0.1	2.2	2.2
20	mean	7.7	3.2	4.6	0.7	41.0	59.0
20	SD	0.4	0.3	0.2	0.0	1.6	1.6
22	mean	14.0	5.6	8.4	0.7	39.9	60.1
22	SD	0.2	0.1	0.2	0.0	1.1	1.1
32	mean	12.0	4.3	7.7	0.6	35.9	64.1
32	SD	0.3	1.3	1.4	0.2	11.3	11.3
34	mean	20.0	7.3	12.7	0.6	36.7	63.3
34	SD	0.8	1.2	1.4	0.1	6.0	6.0
44	mean	17.0	6.8	10.3	0.7	39.7	60.3
44	SD	0.3	0.2	0.3	0.0	1.2	1.2
46	mean	24.0	9.6	14.4	0.7	40.0	60.0
46	SD	0.8	0.5	0.5	0.0	1.2	1.2
56	mean	20.8	8.2	12.6	0.7	39.6	60.4
56	SD	0.6	0.3	0.4	0.0	0.4	0.4
58	mean	28.2	11.2	17.0	0.7	39.8	60.2
58	SD	0.6	0.2	0.4	0.0	0.5	0.5
68	mean	24.0	9.6	14.4	0.7	40.0	60.0
68	SD	0.8	0.4	0.5	0.0	0.5	0.5
70	mean	28.0	11.1	16.9	0.7	39.8	60.2
70	SD	0.8	0.4	0.4	0.0	0.3	0.3
80	mean	24.1	9.7	14.4	0.7	40.3	59.7
80	SD	0.8	0.4	0.5	0.0	0.4	0.4
82	mean	28.8	11.4	17.4	0.7	39.7	60.3
82	SD	0.5	0.3	0.4	0.0	0.7	0.7
92	mean	25.0	10.0	15.0	0.7	40.0	60.0
92	SD	0.6	0.3	0.4	0.0	0.4	0.4
94	mean	27.0	10.7	16.4	0.7	39.4	60.6
94	SD	0.4	0.4	0.2	0.0	1.1	1.1
104	mean	23.0	9.2	13.8	0.7	40.0	60.0
104	SD	0.4	0.1	0.3	0.0	0.3	0.3
106	mean	25.2	10.0	15.1	0.7	39.9	60.1
106	SD	0.6	0.3	0.3	0.0	0.3	0.3
116	mean	21.0	8.4	12.6	0.7	40.0	60.0
116	SD	0.8	0.4	0.5	0.0	0.9	0.9
118	mean	23.2	9.3	13.8	0.7	40.3	59.7
118	SD	0.5	0.3	0.5	0.0	1.2	1.2
128	mean	19.0	7.7	11.4	0.7	40.3	59.7
128	SD	0.3	0.2	0.4	0.0	1.3	1.3
130	mean	26.1	10.3	15.7	0.7	39.6	60.4
130	SD	0.7	0.2	0.6	0.0	0.9	0.9
140	mean	21.9	8.9	13.0	0.7	40.5	59.5
140	SD	0.7	0.3	0.4	0.0	0.5	0.5
142	mean	28.5	11.3	17.2	0.7	39.7	60.3
142	SD	0.6	0.2	0.5	0.0	0.6	0.6
152	mean	25.0	10.1	14.9	0.7	40.3	59.7
152	SD	0.6	0.4	0.2	0.0	0.6	0.6
154	mean	28.1	11.2	16.9	0.7	39.9	60.1
154	SD	0.7	0.3	0.5	0.0	0.6	0.6
164	mean	24.0	9.7	14.3	0.7	40.3	59.7
164	SD	0.9	0.4	0.6	0.0	0.9	0.9
166	mean	27.5	10.9	16.6	0.7	39.6	60.4
166	SD	0.6	0.3	0.4	0.0	0.5	0.5
176	mean	23.4	9.4	14.0	0.7	40.2	59.8
176	SD	0.5	0.2	0.3	0.0	0.3	0.3
178	mean	28.1	11.2	16.9	0.7	39.8	60.2
178	SD	0.6	0.3	0.5	0.0	0.6	0.6

Table 1: Peak and trough serum Phenobarbital levels in normal dogs given oral phenobarbital every 12 hours for 1 week. Levels are expressed in μg/ml for total, free and protein-bound phenobarbital serum levels. Samples were taken before medication and 2 hours following administration of 4 mg/kg dosage. Data are as mean and standard deviation (n=5).