Figure 2: Micrographs through the central retina of healthy (+/-) and dystrophic (-/-) RCS rat eyes (both 12 months of age), stained for alphaBcrystallin and GFAP. During outer retina dystrophy, some staining of alphaBcrystallin appeared at the outer limiting membrane (open arrow), presumably by single migrating astrocytes from the inner layers (star in b). Muller cells showed staining for GFAP but not for alphaB-crystallin. NFL=nerve fiber layer, INL=inner nuclear layer. Scale bar equals 50 μm.