Table 3

S.No	Virus	Gene target	Detection limit in ≤Copy numbers*
S.No	Virus	Gene target	Individual PCR	Multiplex PCR
1.	Porcine circo virus 2	ORF1	1.36×102	5.44×102
2.	Porcine Parvovirus	NS1	1.41×102	5.62×102
3.	African swine fever virus	VP73	7.05×101	2.82×102
4.	Bovine parvo virus	ParVgp1	1.49×101	7.27×101
5.	Bovine herpes virus	UL39	2.66×101	4.44×102
6.	Porcine reproductive and respiratory syndrome virus	ORF7	1.38×102	5.84×102
7.	Classical swine fever virus	NS5B	1.44×102	5.70×102
8.	Swine vesicular disease virus		6.35×101	5.48×102
9.	Bovine Pestivirus	5’UTR	2.12×101	1.36×102
10.	Bovine coronavirus	NS1	1.37×101	5.96×102
11.	Blue tongue Virus	NS1	1.06×102	not included

Table 3: Lower limit of detection of synthetic plasmid constructs in individual and multiplex PCRs. *the formula used for converting the DNA quantities into number of copies = (amount in ngx 6.022×10²³) / (length in bp×1×10⁹ng/g ×650 g/ mole of bp).