Primer Sequence Base Position F1 5’ ATG ATG RGC TGG CAT TCT 3’ (15,257-15,274) F2 5’ ATR ATG RGC TGG CAT TCC 3’   F3 5’ ATG ATG GGC CGG CGT TCC 3’   R1 5’ ACA CGG TCG CCC TAA TTG 3’ (15,353-15,370) Probe 5’HEX-TGT GGT GAA TGG CAC TGA TTG ACA-BHQ2 3’ (15,307-15,330)

Table 1: Primer and probe sequences for the North American strain VR-2332 of PRRSV used to detect viral RNA in tissue samples from infected hogs.