Table 1

Gene¹	Sequence(5’→3’)	AnnealingTemp.(°C)	Productsize(bp)	AccessionNumber
Sox2	F-gcagagaaaagggaaaaagga	48.0	170	HG313861
	R-tttcctagggaggggtatgaa
Sox3	F-tgttcgcttccgagtcttaaa	48.0	231	JQ280473
	R-cctttccgtaggaacaaaacc
Gata-4	F-tgagaaaagagggcattcaga	47.0	262	JN639851
	R-gcaggatgaattgaagatcca
PouV	F-gttgtccgggtctggttct	54.0	189	HE608817
	R-gtggaaaggtggcatgtagac
DMRT	F-gtggcagatgaagggaatggag	57.0	187	AF123456
	R-gctgggcggcgaaaacac
AMH	F-accgtctacgcagccaacaact	57.0	182	HE646741
	R-gccgacacgctgatgatgag
ASW	F-gcctgggcttgaccgatggat	60.0	201	JN942586
	R-ggtggctaggctgacgggcaac
P-450	F-attgcagacgaccccacagtg	59.0	239	J04047
	R-atgcgtccaatgttgagaataatg
28s	F-caggtgcagatcttggtggtagta	58.0	274	JN942581
	R-gctcccgctggcttctcc

¹Sox-2=SRY (sex determine region Y)-box 2, Sox-3=SRY (sex determine regionY)-box 3, GATA-4 =GATA-4 Transcription factor, POU-V=POUV POU domainclass 5 transcription factor, DMRT: Doublesex/Mab-3 Related TranscriptionFactor, AMH: Anti-Mullerian Hormone, ASW: Avian Sex-specific W-linked, P450: Cytochrome P450.

Table 1: Oligonucleotide sequence of pluripotent and sex related genes of turkey.