| Sl no. |
Time intervals of
observation |
Cytopathic effects in CEF from 0 hours PI to 96 hours PI |
| Field virus isolate |
BOM–1 isolate |
| 1 |
0 hour |
No CPE observed |
No CPE observed |
| 2 |
12 hours |
No CPE observed |
No CPE observed |
| 3 |
24 hours |
Rounding of cells, small cytoplasmic vacuolations |
Same CPE but more intense and prominent. |
| 4 |
36 hours |
Variable sizes of plaques, discrete clumping of cells. |
Comparatively larger sizes of plaques and cell clumps. |
| 5 |
48 hours |
Formation of Syncytia, Number and diameter of plaques increased. |
Large cell clumps and plaques but no syncytia observed. |
| 6 |
60 hours |
Mild coalescence of plaques. |
Syncytia and coalescence of plaques. |
| 7 |
72 hours |
large syncytia observed, Disintegration of cell sheet started. |
Syncytia formation, Coalescence of plaques, large areas of cell
destruction and disintegration, |
| 8 |
84 hours |
Large cell clumps, Disorganization of monolayer. |
Disorganization of monolayer and Large cell clumps floating on
superficial media. |
| 9 |
96 hours |
Absence of cellular morphology and cell organization |
Detachment of cell sheet from the adhering surface. |
