Histological
Figure 5: Histological and histochemical analysis of the differentiated stage (30-40 days). Figure 5A: Cross section in the caudal part of the oviduct at 30-days old showing wide lumen and numerous folds. Note the beginning of differentiation of sperm host gland (asterisk). (H & E). Figure 5B: Higher magnification showing the ciliated (cc) and secretory (sc) lining epithelium with beginning of formation of glands at the base of the fold (arrowheads). (H & E). Figure 5C: The epithelium showing negative reaction by PAS (arrowhead). Figure 5D: Alcian blue gave weak positive reaction at the apical portion of the epithelium (arrowhead). Figure 5E: Many elastic fibers which present within the folds and around the blood vessels (arrowheads). (Wighert's Elastica). Figure 5F: In Semithin section at 40-days old, the epithelium showing ciliated (cc) and secretory cells (sc). Note presence of glands (G) that opened to the epithelium by their ducts. (TB).