Sl No. mRT-PCR Primers Primer Name Gene Sequences (5ʺ-3ʺ) Product Size (bp) Reference
1 AIV
(All-M)
AIV- M-F M (Matrix Protein) gene GAA GGT AGA TAT TGA AAG ATG 1,023 Rashid et al. [17]
AIV-M- R GAA ACA AGG TAG TTT TTT ACT C
2 IBV IBV-F 5ʺ UTR (untranslated region ) GCT TTT GAG CCT AGC GTT 149
IBV-R GCC ATG TTG TCA CTG TCT ATT
3 NDV NDV-F F0 (fusion protein) gene GGA GGA TGT TGG CAG CAT T 320
NDV-R GTC AAC ATA TAC ACC TCA TC
4 ILTV ILTV-F Tk (Thimidine Kinase) gene ACG ATG ACT CCG ACT TTC 647
ILTV-R CGT TGG AGG TAG GTG GTA
Table 1: Details of the primers used for multiplex PCR.