Figure 6: Time spent in the center of an open field by ovariectomized rats that received a Silastic tube with and without estradiol. Ovariectomized rats received a Silastic tube implant that was empty (Se) or filled with 3 mg (S 3 mg E) or 4 mg (S 4 mg E) estradiol benzoate and tested in an open field to assess the time spent in the Center box. Time spent in the Center box after (A) 7 days, (B) 14 days, (C) 21 days or (D) 28 days of estradiol treatment. Estradiol treated rats spent more time at the center at 21 days than non-treated rats (Se, Silastic empty) (p=0.009 F(2,25)=5.7550, repeated measures ANOVA followed by Newman-Keuls multiple comparison test). (n=7-8 per group). Data are expressed as mean ± S.E.M.