Figure 2: Sequence from the study and reference sequence obtained from the GenBank aligned using multiple alignment programs Clustal X. Evolutionary distance computed using the Maximum Composite Likelihood. The amount of genetic change is represented by a scale of 0.1. The number of substitution is represented by a percentage value; that is the number of substitution per 100 nucleotide sites and is shown above the branches. The reference sequences are in bold. The tree was rooted to the genus Ixodes .