Genus Accession Number Tick species ITS fragment Size % Identity Reference sequence Origin Country Rhipicephalus       KM819710       R.pulchellus 1100 100 AF271275 Australia R.puchellus 1100 100 AF271275 Australia R.pulchellus 1200 98 AF271275 Australia R.pulchellus 1200 98 AF271275 Australia R.pulchellus 1100 99 AF271275 Australia R.pulchellus 1100 99 AF271275 Australia Amblyomma   KM819712           A.variegatum 1200 98 HQ856759 U.S.A A.variegatum 1200 98 HQ856759 U.S.A A.variegatum 1200 98 HQ856759 U.S.A A.variegatum 1200 98 HQ856759 U.S.A A.variegatum 1200 97 HQ856759 U.S.A A.variegatum 1200 98 HQ856759 U.S.A A.variegatum 1200 98 HQ856759 U.S.A A.humerali   800   91 AY887111   Brazil   Hyalomma               KM819713             H. marginatum 1500 95 JQ737104 China rufipes         H. marginatum 1500 95 JQ737104 China rufipes         H. marginatum 1500 95 JQ737104 China rufipes         H. marginatum 1500 95 JQ737104 China rufipes         H. marginatum 1600 95 JQ737104 China rufipes         H. marginatumrufipes 1600 95 JQ737104 China KM819711 H.dromedarii 1200 95 JQ733570 India H.dromedarii 1200 92 JQ733570 India

Table 2: Identity of tick species and their bp sizes, and percentage similarity value with the references sequences.