Immunization cycles Serum dilutions
1/100 1/200 1/400 1/800 1/1600 1/3200 1/6400
1st IC 0.739 0.620 0.484 0.386 0.315 0.285 0.255
2nd IC 0.824 0.725 0.586 0.465 0.373 0.334 0.314
3rd IC 0.928 0.815 0.679 0.535 0.436 0.365 0.342
4th Sampling 0.792 0.677 0.527 0.408 0.307 0.281 0.212
Negative control(M+3SD)   (M + 3SD) 0.207 0.203 0.191 0.109 0.111 0.097 0.091
Table 2: Mean OD450 values of indirect ELISA of gentamycin antisera(Bold numbers indicate 50 titres).