Immunization cycles Serum dilutions
1/100 1/200 1/400 1/800 1/1600 1/3200 1/6400
1st IC 0.905 0.784 0.59 0.428 0.283 0.094 0.068
2nd IC 1.052 0.97 0.807 0.528 0.358 0.198 0.135
3rd IC 2.072 1.865 1.581 1.324 1.239 1.11 1.041
4th IC 1.458 1.282 0.999 0.84 0.633 0.435 0.25
Negative control
(M+3SD)
0.211 0.207 0.218 0.175 0.115 0.121 0.110
Table 3: Mean OD450 values of indirect ELISA of ceftiofur antisera(Bold numbers indicate 50 titres).