Species Porcine- specific PCR system Bovine- specific PCR system Ovine- specific PCR system Caprine specific PCR system Avian (chicken)-specific PCR system Endogenous control- specific PCR system Capra hircus ND ND ND 22.0± 0.0 ND ND Bostaurus ND 23.0 ± 0.1 ND ND ND ND Ovisaries ND ND 22.0± 0.0 ND ND ND Gallus gallus ND ND ND ND 21.4 ± 0.1 ND Susscrofa 17.8± 0.0 ND ND ND ND ND Zea mays and Glycine max (endogenous controls) ND ND ND ND ND 15.8 ± 0.0

Table 2: Species-specific qPCR systems. Cross-amplification of DNA templates from each species was performed against others by using qPCR species-specific systems. Ct-values (mean and standard deviation) were obtained using 10 ng of each DNA template from animal blood and endogenous controls. Triplicate PCR reactions were run from each DNA template. ND- animal material not detected (no positive signal after 40 qPCR cycles).