| Parts of CYP2D14 gene (length of PCR product) |
Primer for PCR and DNA sequencing |
Tm(°C) |
|
Exon 1-4 |
1L: 5’-TCCCCATCATCAACCTTGTCCTC-3’ |
62 |
| (2.5 kbp:1L+1R) |
1R： 5’-CCTCCGCTCCCTTTATACAGAGG-3’ |
|
| Exon 5-9 |
2L: 5’-CTGGTTTTGGGGAGACTGCTAAG-3’ |
61 |
| (2.0 kbp:2L+2R) |
2R: 5’- GACAACCTAGATCGGAAAGGCTG-3’ |
|
| Complementary strand |
| Exon 1-4 |
3L: 5’-GCCCAGAAAGATAAACCTAAAATGT-3’ |
60 |
| (2.5 kbp:3L+3R) |
3R: 5’-ACTCTCCTTGACCCCTCTCTGTACT-3’ |
|
| Exon 5-9 |
4L: 5’-GTGGGAGTACTTCACTGCAAGG-3’ |
60 |
| (2.0 kbp:4L+4R) |
4R: 5’-AGACACTGGTTTATTGACCATCAG-3’ |
|
| Specific primer for DNA sequencing related to parts of polymorphism |
| Complementary strand |
| Exon 1 |
5L: 5’-AGTCTGTTTCCATGTTGTAAAAACC-3’ |
|
| Exon 3 |
6L: 5’-CGCACTAAGTTAAACTCTTCCTTCA-3’ |
|
| Exon 3 |
5R: 5’-CAAAATAAAAAGTGGAGCCAAGAC-3’ |
|