Pharmacokinetic Parameter GD1 GD2 WT Fisher’s LSD Mean SD Mean SD Mean SD *:P < 0.05 Unit A 825.94 800.32 1,311.49 1,277.36 1,028.76 1,887.75   ng/mL B 76.1 28.67 62.32 23.79 151.84 217.96   ng/mL C 2.62 1.69 1.98 1.76 10.5 17.56   ng/mL α 8.06 2.22 7.53 4.13 3.55 2.25 GD1-WT* h-1 β 0.66 0.27 0.76 0.36 0.86 0.85   h-1 γ 0.07 0.03 0.08 0.04 0.13 0.08   h-1 Cmax 2,404.66 3,505.73 1,305.17 1,301.15 1,189.97 1,909.29   ng/mL Vda 0.99 0.95 0.85 0.54 1.6 1.21   L/kg K21b 2.03 1.45 1 0.22 1.29 1.13   h-1 K31c 0.08 0.04 0.09 0.05 0.3 0.27 GD1-WT* h-1 K10 4.05 2.7 4.1 3.12 2.02 1.42   h-1 K12b 2.19 1.28 1.69 1.3 0.75 0.86   h-1 K13c 0.44 0.41 0.27 0.11 0.28 0.36   h-1 K10HL 0.28 0.15 0.28 0.25 0.53 0.3   h αHL 0.09 0.02 0.25 0.35 0.34 0.24   h βHL 1.17 0.31 1.07 0.28 1.6 1.38   h γHL 12.62 5.73 10.8 5.09 5.91 2.63 GD1-WT* h AUC0-30 202.98 86.59 152.55 42.73 281.97 199.37   ng . h/mL AUC0-infd 392.98 329.5 236.67 86.61 391.27 363.6   ng . h/mL Cle 2.63 2.46 1.89 0.61 1.89 1.13   L/h.kg AUMCf 955.03 532.59 687.71 446.66 586.88 343.14   ng . h2/mL MRTg 3.02 1.41 3.2 2.15 2.06 0.72   h Vssh 5.15 3.45 6.28 4.23 5.01 2.77   L/kg

Table 3: Pharmacokinetic parameters of plasma metoclopramide in gene deletion 1 (GD1), gene deletion 2 (GD2) and wild –type (WT) cattle after IV administration of metoclopramide at 0.4 mg/kg (mean ± standard deviation (SD), n=6). aVd: Volume of the central compartment. bK12 and K21: Transfer constant from central (1) to peripheral (2) and from 2 to 1, respectively. cK13 and K31: Transfer constant from central (1) to deep peripheral (3) and from 3 to 1, respectively. dAUC0-inf: Estimated area under the concentration-time curve from 0 to infinity. eCl: Total body clearance. fAUMC: Area under the first moment curve. gMRT: Mean residence time. hVdss: Volume of distribution at steady-state.