Pharmacokinetic parameter GD1 GD2 WT Fisher’s LSD   Mean SD Mean SD Mean SD *:P<0.05 Unit **:P<0.01  A 278.32 414.5 24.47 43.03 112.6 222.38   ng/mL B 6.45 1.15 6.43 2.66 5.24 1.42   ng/mL α 2.07 1.65 2.45 1.98 2.05 2.65   h-1 β 0.06 0.01 0.06 0.02 0.06 0.02   h-1 Cmax 9.2 1.76 6.12 1.62 4.09 0.35  GD1-GD2* ng/mL  GD1-WT** Tmax 1.13 0.63 1.63 1.11 1.25 1.19   h Vda 11.13 8.81 25.04 12.35 21.11 11.94   L/kg K21b 0.82 0.6 1.83 1.76 0.94 0.62   h-1 K01c 2.82 1.51 5.04 6.41 4.42 2.21   h-1 K10d 0.16 0.04 0.08 0 0.1 0.04 GD1-GD2** h-1 GD1-WT* K12b 1.15 1.08 0.59 0.58 1.07 2.05   h-1 K01HL 0.35 0.27 0.33 0.24 0.21 0.14   h K10HL 4.13 0.8 8.77 0.49 7.99 3.83 GD1-GD2* h αHL 0.55 0.38 0.45 0.3 0.81 0.59   h βHL 11.11 1.95 12.49 4.11 11.92 3.3   h AUC0-30 98.23 26.89 87.79 21.01 72.13 16.68   ng・h/mL AUC0-infe 107.36 30.16 100.31 18.68 86.72 21.1   ng・h/mL Clf 3.9 1.31 4 0.7 4.73 1.39   L/h・kg AUMCg 1462.74 478.61 1564.32 307.97 1336.17 629.19   ng・h2/mL MRTh 13.46 1.54 15.96 3.97 14.79 3.88   h F0-30 61.19 28.11 66.99 24.02 45.4 29.23   % F0-infi 38.87 23.82 48.46 20.27 44.37 25.65   %

Table 4: Pharmacokinetic parameters of plasma metoclopramide in gene deletion 1 (GD1), gene deletion 2 (GD2) and wild-type (WT) cattle after oral administration of metoclopramide at 0.4 mg/kg (mean ± standard deviation (SD), n=4). aVd: Volume of the central compartment. bK12 and K21: Transfer constant from central (1) to peripheral (2) and from 2 to 1, respectively. cK01: Absorption rate. dK10: Elimination rate. eAUC0-inf: Estimated area under the concentration-time curve from 0 to infinity. fCl: Total body clearance. gAUMC: Area under the first moment curve. hMRT: Mean residence time. iF0-inf: Bioavailability from 0 to infinity.