RNA quantity (fg/ul) (mean/sd) Directional fold change / p value   1 dph 16 wk 16 wk v 1 dph F v RBC2 Gene F RBC2 F RBC2 F RBC2 Combined 1 dph 16 wk Combined TNNC1 45.450 28.200 37.900 47.300 -1.20 1.67 1.16 1.61 -1.25 1.10   23.350 6.000 7.000 5.050 0.65 0.02* 0.49 0.42 0.15 0.64 TNNI1 15.100 8.400 0.510 0.020 -29.71 -430.25 -44.54 1.80 26.01 1.85   10.200 4.725 0.800 0.002 0.13 0.09 0.02* 0.41 0.40 0.47 TNNT2 6.450 7.250 12.900 11.800 2.00 1.64 1.82 -1.12 1.09 1.01   2.695 1.060 1.905 6.500 0.04* 0.35 0.03* 0.67 0.81 0.96 HPRT1 0.487 0.491 0.685 0.695 1.41 1.41 1.41 -1.01 -1.01 -1.01   0.169 0.053 0.088 0.157 0.17 0.17 0.01* 0.97 0.93 0.94

Table 5: Summary of qRT-PCR results. For each gene the mean quantity of RNA (fg/ul) in each qRT reaction is as determined through normalization to PLN. Directional fold changes and p values are given for each comparison. Significant P values (< 0.05; Ho = no difference) are indicated by asterisks.