Time
(min)

0.1% TFA in water

ACN

Mode

    1. → 7.0

70 → 10

30 → 90

Linear gradient

  7.0 → 10.0

           10 → 0

  90 → 100

Linear gradient

        10.0 → 13.0

0

100

Isocratic

 13.0 → 13.01

0 → 70

100 → 30

Sharp gradient

      13.01 → 17.00

70

30

Reequilibrium

Table 1: Optimized HPLC method for assessing imipramine in in vitro samples.