M1

M2

M3

 

Vm

Km

Vm

Km

Vm

Km

CYP 3A4

0.04 ± 0.006

40.1 ± 16.1

0.032 ± 0.003

10.5 ± 4.8

0.20 ± 0.02

57.5 ± 14.7

CYP 3A5

0.025 ± 0.003

10.1 ± 5.1

0.021 ± 0.002

1.5 ± 1.8

0.16 ± 0.01

14.5 ± 2.7

Vm: μM/min/ pmol CYP
Km: μM
Table 2: Kinetic parameters for 17-OHPC hydroxylated metabolites (M1-M3) in expressed CYP3A4 and CYP3A5. All the experiments were performed in triplicate and the values are reported as mean ± SD.