Figure 3: Altholactone inhibits cell proliferation of DU145 cells. The androgen-independent (DU-145) prostate cancer cells were seeded into 96-well plates and incubated overnight. Further, 2h early, we pre-treated cells with N-acetylcysteine, prior addicting of altholactone in a dose dependent manner for 24h supplementary, and then analyzed for cell growth using MTT assay. Data are expressed as Mean ± SD of three independent experiments. Altholactone inhibited cell growth and induced cell death in DU145 cells compared to the control cells (A). N-acetylcysteine inhibits altholactone-induced cell inhibition in human prostate cancer DU145 cells (B-C).