Figure 6: Effect of altholactone on the expression of Bax and Bcl-2, the major apoptosis regulatory proteins. DU145 cells were treated with 0, 20, 30 and 40 μM of altholactone for 24h. Equal amounts of whole-cell extracts were extracted from 10% SDS-polyacrylamide gels transferred to polyvinylidene difluoride (PVDF). Expression levels of Bax, Bcl-2 and β-actin were monitored by western blotting. β-actin antibody was used to verify equal protein loading. Data are representative of two individual experiments with parallel results.